|
KMID : 0357319850200020247
|
|
Journal of the Korean Society for Microbiology
1985 Volume.20 No. 2 p.247 ~ p.255
|
|
|
The Effects of Korea Ginseng, Deer Horn Extracts and Filtrate of Escherichia coli Culture on Leukocyte Chemotaxis
|
|
|
|
|
Abstract
|
|
|
Nuclepore membrane filter assay was introduced by Snyderman and Pike in 1978. This is the most widely used technique for the studying chemotactic activities of which an economic and sensitive test system measured at random and the directional migration of human polymorphonuclear leukocytes and monocytes made a description.
The technique, based on a modified Boyden(1962) and Wilkinson(1974) chamber equipped with filte.(chemotactic chamber by Neuro Probe Inc.) systems, permits a substantial reduction of both incubation and chemotactic factors.
In other. hands, the chemotactic activities of the leukocytes were measured the treatment by filtrate of coli (ATCC 25922 strain) culture, Ginseng extracts and deer horn extracts.
The leukocytes were separated from heparinized whole blood of healthy donor in siliconized tubes made a used of Ficoll-Hypaque solution. The leukocytes were washed out three times with TC-199 medium, pH7.4. The concentration of the leukocyte was at 1.5X10´/ml.
The filtrated bacterial fluid(E. colt), Ginseng and deer horn extracts were used as chemotactic factors with modified Boyden chamber (type B-322) that Nuclepore filters were 5.0 um, 13 mm in diameter. The chambers were filled with human leukocytes and the various chemo attractants were kept at 37C. Then filters removed and stained them with Mayer´s hematoxylin solution and migrated leukocytes counted only twenty fields through the Olympus microscope.
The results obtained were as follows
1. The used chemotactic fractures such as TC-199 medium, milk, filtrate of E. coli culture, Ginseng and Deer horn extracts were effective for migration of the leukocytes but TC-199 medium was shown only poor.
2. The various concentrations of the chemotactic factors such as Ginseng extract (0.1%, 0.05% and 0.01%) were stimulated for the leukocytes migration in the modified Boyden chambers, However, lowest concentration, 0.01% was more effective than others for the chemoattractant of the leukocytes migration.
3. The chemotactic activities that tried for the leukocytes treated with Ginseng extracts (0.1%, 0.05% and 0.01%) showed poorly effect at 37C for 60 minutes.
4. The chemotactic activity of the deer horn extract, 0.1%, was more effective than 0.5%Q and/or 1.0 concentrations for the leukocytes migration.
For these reasons, it has found that Ginseng and deer horn extracts were stimulated for the leukocytes migration in vitro tests.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|